Composite
SAHS 1

Part:BBa_K2623015:Design

Designed by: Jun Dai   Group: iGEM18_XMU-China   (2018-09-28)


Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 279
    Illegal XhoI site found at 503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1152
    Illegal AgeI site found at 1264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 558
    Illegal SapI.rc site found at 553


Design Notes

We used T4 DNA ligase to construct the circuit.



Source

This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini)

References